David, Samuel PhD
1650 Cedar Ave., Montreal, H3G 1A4
My laboratory is interested in three areas: (1) CNS autoimmune demyelinating disease Multiple sclerosis (MS). We have recently characterized the role of the phospholipase A2 (PLA2) super-family in a widely used mouse model of MS called experimental autoimmune encephalitis (EAE). We are currently studying the molecular mechanisms underlying iron accumulation in the CNS in relapsing-remitting and chronic forms of EAE and MS, and the role of iron in disease pathogenesis. (2) The contribution of inflammation (macrophage/microglia) in secondary tissue damage after spinal cord injury. We are particularly interested in characterizing the complex responses of these key cells involved in the innate immune response to CNS injury. (3) Iron homeostasis in the CNS. We are studying the molecular control of iron homeostasis in different cell types in the CNS. This is an important area of work as dysregulation of iron evels contributes to various neurodegenerative diseases such as MS and ALS.
Cell biology, molecular and biochemical techniques, in vivo models, cell culture, anatomical and histological techniques, behavioral analysis of locomotor function.
- Kwon BK, Streijger F, Hill CE, Anderson AJ, Bacon M, Beattie MS, Blesch A, Bradbury EJ, Brown A, Bresnahan JC, Case CC, Colburn RW, David S, Fawcett JW, Ferguson AR, Fischer I, Floyd CL, Gensel JC, Houle JD, Jakeman LB, Jeffery ND, Jones LA, Kleitman N, Kocsis J, Lu P, Magnuson DS, Marsala M, Moore SW, Mothe AJ, Oudega M, Plant GW, Rabchevsky AS, Schwab JM, Silver J, Steward O, Xu XM, Guest JD, Tetzlaff W. Large animal and primate models of spinal cord injury for the testing of novel therapies. Exp Neurol. 2015 Jul;269:154-68. doi: 10.1016/j.expneurol.2015.04.008..
- Jiang R, Hua C, Wan Y, Jiang B, Hu H, Zheng J, Fuqua BK, Dunaief JL, Anderson GJ, David S, Vulpe CD, Chen H. Hephaestin and ceruloplasmin play distinct but interrelated roles in iron homeostasis in mouse brain. J Nutr. 2015 May;145(5):1003-9. doi: 10.3945/jn.114.207316.
- Zarruk JG, Berard JL, Passos Dos Santos R, Kroner A, Lee J, Arosio P, David S. Expression of iron homeostasis proteins in the spinal cord in experimental autoimmune encephalomyelitis and their implications for iron accumulation. Neurobiol Dis. 2015 Feb 25. pii: S0969-9961(15)00021-2. doi: 10.1016/j.nbd.2015.02.001. In press.
- Lim TK, Shi XQ, Johnson JM, Rone MB, Antel JP, David S, Zhang J. Peripheral nerve injury induces persistent vascular dysfunction and endoneurial hypoxia, contributing to the genesis of neuropathic pain. J Neurosci. 2015 Feb 25;35(8):3346-59. doi: 10.1523/JNEUROSCI.4040-14.2015.
- David, S. and Popovich, P.G (2014). Microglial responses to spinal cord and brain trauma. In: Microglia. In: Microglia in health and disease (Eds. Marie-Ève Tremblay and Amanda Sierra), Springer.
- Kroner A, Greenhalgh AD, Zarruk JG, Passos Dos Santos R, Gaestel M, David S. TNF and increased intracellular iron alter macrophage polarization to a detrimental M1 phenotype in the injured spinal cord. Neuron. 2014 Sep 3;83(5):1098-116. doi: 10.1016/j.neuron.2014.07.027.
- Lemmon VP, Ferguson AR, Popovich PG, Xu XM, Snow DM, Igarashi M, Beattie CE, Bixby JL; MIASCI Consortium. Minimum information about a spinal cord injury experiment: a proposed reporting standard for spinal cord injury experiments. J Neurotrauma. 2014 Aug 1;31(15):1354-61. doi: 10.1089/neu.2014.3400.
- Gresle MM, Schulz K, Jonas A, Perreau VM, Cipriani T, Baxter AG, Miranda-Hernandez S, Field J, Jokubaitis VG, Cherny R, Volitakis I, David S, Kilpatrick TJ, Butzkueven H. Ceruloplasmin gene-deficient mice with experimental autoimmune encephalomyelitis show attenuated early disease evolution. J Neurosci Res. 2014 Jun;92(6):732-42. doi: 10.1002/jnr.23349.
- Greenhalgh AD, David S. Differences in the phagocytic response of microglia and peripheral macrophages after spinal cord injury and its effects on cell death. J Neurosci. 2014 Apr 30;34(18):6316-22. doi: 10.1523/JNEUROSCI.4912-13.2014.