Prat, Alexandre MD, PhD

Assistant Professor

Université de Montréal
Hôpital Notre-Dame - CHUM
1560 Sherbrooke Street East, Montréal, QC H2L 4M1
(514) 890-8000 (24734)
(514) 412-7668

Research Profile

Part of our research team focuses on the immunological roles of CD4+T helper lymphocytes in MS, the mechanisms of monocytes and lymphocyte migration across the BBB and the physiological regulation of the BBB by glial cells. Other members of the team study the role of novel tight junction molecules of the human BBB and the influence of glial cell factors on junction formation. We believe that a better knowledge of the molecular composition of the BBB will lead to the understanding of diseases such as MS, as well as to the discovery of novel routes for delivery of drugs and chemotherapies for the treatment of neurodegenerative diseases and brain tumors.

Expertise

Immunobiology, cell biology and pharmacology of the human Blood brain barrier. Immunobiology of the BBB in multiple sclerosis. Astrocytes – brain microvascular endothelium interactions in the human brain. Molecular phenotyping of BBB tight junction in the human brain. Regulation of TJ formation in the human brain(supported by the MSSC). Characterization of dendritic cell population within the human brain. Identification of the molecular signals provided by BBB endothelium to promote the differentiation, maturation and migration of DC within the human brain. Identification of the clinical, radiological and immuno-biological pronostic factors involved in the conversion of the clinically isolated syndromes into clinically definite multiple sclerosis. (supported by the FRSQ)

Recent Publications

  • Larochelle C, Lécuyer MA, Alvarez JI, Charabati M, Saint-Laurent O, Ghannam S, Kebir H, Flanagan K, Yednock T, Duquette P, Arbour N, Prat A. Melanoma cell adhesion molecule-positive CD8 T lymphocytes mediate central nervous system inflammation. Ann Neurol. 2015 Jul;78(1):39-53. doi: 10.1002/ana.24415.
  • Podjaski C, Alvarez JI, Bourbonniere L, Larouche S, Terouz S, Bin JM, Lécuyer MA, Saint-Laurent O, Larochelle C, Darlington PJ, Arbour N, Antel JP, Kennedy TE, Prat A. Netrin 1 regulates blood-brain barrier function and neuroinflammation. Brain. 2015 Jun;138(Pt 6):1598-612. doi: 10.1093/brain/awv092.
  • Legroux L, Pittet CL, Beauseigle D, Deblois G, Prat A, Arbour N. An optimized method to process mouse CNS to simultaneously analyze neural cells and leukocytes by flow cytometry. J Neurosci Methods. 2015 May 30;247:23-31. doi: 10.1016/j.jneumeth.2015.03.021.
  • Michel L, Larochelle C, Prat A. Update on treatments in multiple sclerosis. Presse Med. 2015 Apr;44(4 Pt 2):e137-51. doi: 10.1016/j.lpm.2015.02.008.
  • Duquette P, Prat A. How safe is natalizumab during pregnancy? Mult Scler. 2015 Feb;21(2):121-2. doi: 10.1177/1352458514555789.
  • Alvarez JI, Saint-Laurent O, Godschalk A, Terouz S, Briels C, Larouche S, Bourbonnière L, Larochelle C, Prat A. Focal disturbances in the blood-brain barrier are associated with formation of neuroinflammatory lesions. Neurobiol Dis. 2015 Feb;74:14-24. doi: 10.1016/j.nbd.2014.09.016.
  • Daneman R, Prat A. The blood-brain barrier. Cold Spring Harb Perspect Biol. 2015 Jan 5;7(1):a020412. doi: 10.1101/cshperspect.a020412.
  • Larochelle C, Grand'maison F, Bernier GP, Latour M, Cailhier JF, Prat A. Thrombotic thrombocytopenic purpura-hemolytic uremic syndrome in relapsing-remitting multiple sclerosis patients on high-dose interferon β. Mult Scler. 2014 Nov;20(13):1783-7. doi: 10.1177/1352458514523692.
  • Mayo L, Trauger SA, Blain M, Nadeau M, Patel B, Alvarez JI, Mascanfroni ID, Yeste A, Kivisäkk P, Kallas K, Ellezam B, Bakshi R, Prat A, Antel JP, Weiner HL, Quintana FJ. Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation. Nat Med. 2014 Oct;20(10):1147-56. doi: 10.1038/nm.3681..
  • Aubé B, Lévesque SA, Paré A, Chamma É, Kébir H, Gorina R, Lécuyer MA, Alvarez JI, De Koninck Y, Engelhardt B, Prat A, Côté D, Lacroix S. Neutrophils mediate blood-spinal cord barrier disruption in demyelinating neuroinflammatory diseases. J Immunol. 2014 Sep 1;193(5):2438-54. doi: 10.4049/jimmunol.1400401.
  • Miyazaki Y, Li R, Rezk A, Misirliyan H, Moore C, Farooqi N, Solis M, Goiry LG, de Faria Junior O, Dang VD, Colman D, Dhaunchak AS, Antel J, Gommerman J, Prat A, Fillatreau S, Bar-Or A; CIHR/MSSC New Emerging Team Grant in Clinical Autoimmunity; MSSRF Canadian B cells in MS Team. A novel microRNA-132-surtuin-1 axis underlies aberrant B-cell cytokine regulation in patients with relapsing-remitting multiple sclerosis. PLoS One. 2014 Aug 19;9(8):e105421. doi: 10.1371/journal.pone.0105421. eCollection 2014.